HALAL ACCREDITATION
CERTIFICATE

Laboratory Performing Halal Testing

MIKROKIM FOOD ANALYSIS & CONTROL LABORATORY

Adress : İçören Mah. Değirmenyolu Cad. Kutay İş Merkezi C Blok No:16 Kat:2
Daire:6 Kat:4 Daire:9-10 Ataşehir /İSTANBUL/TÜRKİYE

Accredited in accordance with the OIC/SMIIC 35:2020
Standard for the attached scope.

Halal Accreditation Number

2024-057

Halal Accreditation Scope

Shown in annex

Initial Halal Accreditation Date

07.06.2024

Expiry Date

07.06.2029

This certificate is valid with the halal accreditation scope until the expiry date, as long as the laboratory fully
complies with the OIC/SMIIC 35:2020 Standard, national legislation and Halal Accreditation Agency rules.


HALAL ACCREDITATION SCOPE

Testing Fields-Tested Materials/Products Name of the Test Testing Method (national, international standards, in-house methods)

Meat and Meat Products

Moisture Determination

AOAC 950.46

Animal Feed

Moisture Determination

29955 Official Gazette No. (Regulation on sampling and analysis methods for the official control of feed)

Cheese

Moisture Determination

AOAC 948.12

Butter

Moisture Determination

AOAC 920.116

Cereal and Cereal Products (Except Corn and Legumes)

Moisture Determination

TS EN ISO 712

Bread

Moisture Determination

TS 5000

Roasted, Ground Coffee

Moisture Determination

TS ISO 11294

Instant Coffee

Moisture Determination

TS ISO 3726

Food (Ash ContentCovers Foodswith Ash Amount in 100 Grams 0,07to 8 Grams.)

Determination of Ash

NMKL 173

Feed and Feed Additives

Determination of Ash

AOAC 942.05

Meat and Meat Products

pH
Electrometric Method

TS 3136 ISO 2917

Instant Coffee

pH
Electrometric Method

TS ISO 3726

Bread and Biscuit

Determination of Salt
Titrimetric Method

TS 5000

Meat and Meat Products

Determination of Salt
Titrimetric Method

AOAC 935.47

Meat and Meat Products

Determination of Starch
Titrimetric Method

TS 6812

Food

Determination of Total Nitrogen and Protein
Kjeldahl Method

NMKL No:6

Feed and Feed Additives

Determination of Total Nitrogen and Protein
Kjeldahl Method

AOAC 2001.11

Food
(Except Milk and Dairy Products)

Determination of Fat Content

NMKL 160

Milk and Milk Products

Determination of Fats by Gerber Method

In-house Method-“MİKROKİM.SOP.02.165 Rev01”(Modified from ButyrometricDetermination of Fat Content of VariousDairy Product)

Feed and Feed Additives

Determination of Crude Oil or Ether Extract

AOAC 920.39

Honey

Sugar Components (Glucose, Fruktose, Saccarose, Lactose and Maltose) Analysis
HPLC-RID Dedector

IHC Bölüm 7.2

Chocolate

Sugar Components (Glucose, Fruktose, Saccarose, Lactose and Maltose) Analysis
HPLC-RID Dedector

AOAC 980.13

Food

Determination of MonosodiumGlutamate (MSG) (in Terms of L-GlutamicAcid)
Spectrophotometric Method

In-house Method-"MİKROKİM.SOP.02.026 REV.03"

(Modified from R-Biopharm L-GlutamicAcid Colorimetric Method)

Fish and Fish Products

Determination of Biogenic Amines (Histamine)
HPLC - UV Method

NMKL 196

Food

Colour / Determination of Food Dyes
(E - 102 Tartrazin / Acid Yellow 23,
E - 104 Quinoline Yellow,
E - 110 Sunset Yellow FCF,
E - 120 Carminicacid / Acid Red 4,
E - 122 Carmosin (Azorubin),
E - 123 Amaranth / Acid Red 27,
E - 124 Ponceau 4R / Acid Red 18,
E - 127 Eritrosin B,
E- 129 Allura Red AC,
E -131 Patent Blue V / Acid Blue 3,
E - 132 Indigotine / Indigo Carmine,
E - 133 Brillant Blue FCF / Acid Blue 9, E - 142 Green S / Acid Green 50)
HPLC - UV / DAD Method

In-house Method-"MİKROKİM.SOP.02.177 REV.01"

(Modified from NMKL 130, WatersApplication Note)

Gıda, Gıda Takviye

Asesülfam-K, Aspartam ve Sakkarin Tayini
HPLC–UV /DAD Metodu

JAOAC Vol.76 No:2 TS EN 12856

Food, Food Supplement

Determination of Acesulfame-K, Aspartame and Saccharin
HPLC–UV /DAD Method

JAOAC Vol.76 No:2 TS EN 12856

Food, Aroma and Food
Reinforcement

Determination of Benzoic Acid and Sorbic Acid
HPLC-UV/DAD Method

NMKL 124

Tea, Coffee and Soft Drinks

Determination of Caffeine
HPLC–UV/DAD Method

JAOAC Vol.76 No:2

Food

Determination of PHB (Methyl-Ethyl and Propyl P-Hydroxybenzoate)
HPLC-UV/DAD Method

NMKL 124

Food

Determination of Antioxidant (BHA -BHT)
 HPLC-DAD Method

İşletme İçi Metot “MİKROKİM.SOP.02.201 Rev01" (AOAC 983. 15’ten Modifiye Edilmiştir.)

Jelatine

Determination of Hydrogen Peroxide

European Pharmacopoeia 8.0 Gelatine Sayfa 2323, 91312 Quantofix Peroxid 100

Meat and Meat Products

Determination of Collagen and Hydroxyproline
Spectrophotometric Method

AOAC 990.26 
 TS 6236 ISO 3496

Animal Fats and Milk and Dairy Products

Search for Vegetable Oil
GC-FID Method

Turkish Food Codex 2017/26Communique on Olive Oil and PirinaOil Analysis Methods
TS 7503

Animal and Vegetable Oils, Extracted Oils

Determination of Fatty Acid Composition - Trans fats, Saturated fatty acids-Unsaturated fatty acidsmonounsaturated fatty acids-Polyunsaturated fatty cids(Buturic Acid (C4:0), Caproic acid (C6:0), Caprylic Acid (C8:0), Capric acid(C10:0), Undecanoic Asit (C11:0), Laurik Acid(C12:0), Tridecanoic Acid(C13:0), Miristikc Acid (C14:0), Miristoleik Acid (C14:1), Pentadecanoic Acid(C15:0), Cis-10 - Pentadecanoic Acid (C15:1), Palmitik Acid (C16:0),Palmitoleik Acid (C16:1), Heptadekanoik Acid (C17:0), Heptadesenoik Acid(C17:1), Stearik Acid (C18:0), Elaidic Acid (C18:1n9t), Oleik acid (C18:1n9c),Linolelaidic acid (C18:2n6t), Linoleic acid (C18:2n6c), Araşidic Acid (C20:0),γ-Linolenic asid (C18:3n6), Gadoleik Asit (C20:1), Linolenic asid (C18:3n3),Heneicosanoic acid (C21:0), Cis-11,14-Eicosadienoic Acid (C20:2), BehenikAcid (C22:0), Cis- 8,11,14 -Eicosatrienoic Acid (C20:3n6), Erusik Acid(C22:1n9), Cis-11,14,17 -Eicosatrienoic Acid (C20:3n3), Tricosanoic Acid(C23:0), Arachidonic Acid (C20:4n6), Cis-13,16 -Docosadienoic Acid (C22:2),Lignoserik Acid (C24:0), Cis-5,8,11,14,17 -Eicosapentaenoic Acid (C20:5n3),Nervonik Acid (C24:1) Cis- 4,7,10,13,16,19-Docosahexaenoic Acid
GC-FID Method

TGK 2017/26 Communique on OliveOil and Pirina Oil Analysis Methods In-house Method-” MİKROKİM.SOP.02.179 Rev00”Modified from Agilent ApplicationNote, Analysis of Fatty Acids

Non-Alcoholic Beverages

Determination of Ethanol (Ethyl Alcohol) and Methanol (Methyl Alcohol)
GC FID Method

AOAC 983.13
In-house Method-“MİKROKİM.SOP.02.167 Rev02”(Modified from Alcohols Fast Analysisof Alcohols in Alcoholic BeveragesAgilent App. Note)

Spice

Determination of Aflatoxin B and Total Aflatoxin (B+B+G+G)
HPLC-FLD Method

AOAC 999.07

Dried Fruits

Determination of Aflatoxin B and Total Aflatoxin (B+B+G+G)
HPLC-FLD Method

AOAC 999.07

Cereals and Cereal Products

Determination of Aflatoxin B and Total Aflatoxin (B+B+G+G)
HPLC-FLD Method

AOAC 991.31

Milk, Dairy Products, Milk-BasedBaby Food

Determination of Aflatoxin M
HPLC-FLD Method

TS EN ISO 14501
In-house Method-"MİKROKİM.SOP.02.160 Rev03"(Modified from R-Biopharm MilkExtraction Method for Aflatoxin M1 RefNo: A11-RP71 / 70N.V2)

Cereal Based Baby and Child Foods

Determination of Aflatoxin B and Total Aflatoxin (B+B+G+G)
HPLC-FLD Method

AOAC 2000.16

Oil Seeds and Products

Determination of Aflatoxin B and Total Aflatoxin (B+B+G+G)
HPLC-FLD Method

İşletme İçi Metot -" MİKROKİM.
SOP.02.128 Rev No:4" (AOAC 991.31
standartdan modifiye edilmiştir.)

Feed (Mixed, Bovine, Poultry Feed,Cat-Dog Food)

Determination of Aflatoxin B and Total Aflatoxin (B+B+G+G)
HPLC-FLD Method

AOAC 2003.02

Cereals, Cereal Products, CerealBased Baby and ChildSupplementary Foods

Determination of Deoxynvalenol (DON)
HPLC UV Method

In-house Method-"MİKROKİM.SOP.02.158 Rev02"(Modified from R-Biopharm BabyfoodDeoxynivalenol extraction method Refno: A2 -P50.V8 / R-Biopharm CerealDeoxynivalenol Extraction Method Refno: from A1 -P50.V10)

Feed

Determination of Deoxynvalenol (DON)
HPLC UV Method

In-house Method-"MİKROKİM.SOP.02.066 Rev00"(Modified from R-Biopharm AnimalFeed Deoxynivalenol Extraction Method)

Corn and Corn Containing Products, Corn Containing Baby Foods

FDetermination of Fumonisin (FB+FB)
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.155 Rev02" (R-Biopharm-Fumonisin Extraction MethodRef. No: P31 / V9 / 16.07.12 AOAC2001.04

Chocolate and Cocoa

Determination of Ochratoxin A
HPLC-FLD Method

R-Biopharm, Cereal Ochratoxin A

Spices

Determination of Ochratoxin A
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.184 Rev00" R-biopharm Ochraprep Analysis of SpiceA20 / P14 V.9

Coffee and Coffee Products

Determination of Ochratoxin A
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.134 Rev04"(Modified from R-Biopharm OchratoxinA App. Note Coffee, Ref Code: A1-P14.V9)

Cereal Based Baby and Kids Foods

Determination of Ochratoxin A
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.082 Rev03" (R-Biopharm Ochratoxin A Method BabyFood)

Cereals and Cereal Products

Determination of Ochratoxin A
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.133 Rev04"(Modified from R-Biopharm Rhone Ltd.Application Note Ochratoxin AExtraction Method Cereal P 14 Ref No: V16)

Feed

Determination of Ochratoxin A
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.118 Rev03"(Modified from R-Biopharm OchraprepAnalysis of Animal Feed A18-P14.V7)

Fruit Juices and Purees Containing Apple, Fruit-Based Baby and Kids Foods

Determination of Patulin
HPLC-UV Method

In-house Method-"MİKROKİM.SOP.02.104 Rev03"(Modified from R-Biopharm EAISIMIPPatulin P250 / P250B, Apple Puree)

Cereals, Cereal Products, CerealBased Baby and ChildSupplementary Foods

Determination of Zearalenon (ZON)
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.157 Rev02"(Modified from R-Biopharm BabyfoodZearelenone extraction method Ref no: A4 -RP91.V1 R-Biopharm CerealZearelenone Extraction Method Ref: A4-RP91 RP90.V13)

Feed

Determination of Zearalenon (ZON)
HPLC-FLD Method

In-house Method-"MİKROKİM.SOP.02.079 Rev02"(Modified from R-Biopharm inZearalenone Analysis of Animal FeedRP91 / RP90 / v15)

Food and Food Additiviesi

Lead (Pb), Cadmium (Cd), Arsenic (As), Nickel (Ni),Crom (Cr),Cobalt(Co),Antimony (Sb), Calcium (Ca),Magnesium (Mg),Potassium(K),Phosphorus (P), Sodium (Na),Mercury (Hg), Iron (Fe), Copper (Cu), Zinc(Zn), Manganese (Mn), Selenium (Se), Aluminium (Al),
ICP MS Method

In-house Method-“MİKROKİM.SOP.02.238” (NMKL186, Modified)

Food and Food Additiviesi

Tin (Sn) Analysis
ICP-MS Method

NMKL 191

Feed and Feed Additivies

Lead (Pb), Cadmium (Cd),Arsenic (As), Nickel (Ni),Crom (Cr),Cobalt(Co),Antimony (Sb), Calcium (Ca),Magnesium (Mg),Potassium(K),Phosphorus (P), Sodium (Na),Mercury (Hg), Iron (Fe), Copper (Cu), Zinc(Zn), Manganese (Mn), Selenium (Se), Aluminium (Al)
ICP MS Method

In-House Method-“MİKROKİM.SOP.02.236" (NMKL186, Modified)

Feed and Feed Additivies

Tin (Sn) Analysis
ICP-MS Method

NMKL 191

Food Contanct
Polymer
,Silicone,Paper/Board Based Food Packaging Materials and Materials

Determination of Structure
FT-IR Method

In-House Method-
“MİKROKİM.SOP.02.153 Rev03” (Modified from Polymer Data HandBook)

Plastic Materials and Materials to beused for Children, PolycarbonateType Plastics, Plastic BasedMaterials in Contact with Food

Specific migration
[2,2-bis(4-hydroxyphenyl) propane (Bisphenol A)]
Analysis
HPLC-FLD Method

EN 14372
TS EN 14372

Plastic Materials and Articles inContact with Foodstuffs

Total Migration Analysis in Evaporative Food Simulants
Method 1-Full Immersion Method

TS EN 1186-1, TS EN 1186-3 Metot 1

Plastic Materials and Articles inContact with Foodstuffs

Total Migration Analysis in Evaporative Food Simulants
Method 3-Bag Method


TS EN 1186-1, TS EN 1186-3 Metot 3

Plastic Materials and Articles inContact with Foodstuffs

Total Migration Analysis in Evaporative Food Simulants
Method 4-Reverse Bag Method

TS EN 1186-1, TS EN 1186-3 Metot 4

Plastic Materials and Articles inContact with Foodstuffs

Total Migration Analysis in Evaporative Food Simulants
Method 5-Filling Method


TS EN 1186-1,TS EN 1186-3 Metot 5

Plastic Materials and Articles inContact with Foodstuffs

Overall Migration Analysis High Temperature Substitution Method (FattyFoods) Modified Polyphenylene Oxide (MPPO)

TS EN 1186-1                                         
   TS EN 1186 - 13

Plastic Based Substances andMaterials in Contact with Food

Specific Migration
Cadmium (Cd), Nickel (Ni), Cobalt (Co), Antimony (Sb), Iron (Fe), Copper(Cu), Zinc (Zn), Manganese (Mn), Aluminium (Al), Terbium (Tb), Europium(Eu), Barium (Ba), Lithium (Li), Gadalonium (Gd), Lantanium (La)
ICP-MS Method

TS EN 13130-1

Dyes Used in Plastics Food ContactMaterias

Lead (Pb), Cadmium (Cd), Arsenic (As), Mercury (Hg), Antimony (Sb),Barium (Ba), Selenium (Se), Chromium (Cr)
ICP-MS Method

NMKL 191
In-House Method-"MİKROKİM.SOP.02.236" (AP (89)1,NMKL 186, Modified)

Paper and Board and PlasticSubstances and Materials in Contactwith Food

Arsenic (As),Mercury (Hg),Kadmium (Cd),Lead (Pb)
ICP-MS Method

TS EN 645,
TS EN 647,
In-House Method-“MİKROKİM.SOP.02.237” (TS 12498Modified)

Porcelain, Ceramic and Glass BasedMaterials in Contact with Food

Determination of Lead (Pb) and Cadmium (Cd) Release
ICP-MS Method

TGK 2012/30

Printed Paper and Board and Materials in Contact with Food

Determination of Chloride (Cl2)
Titrimetric Method

Mohr Method TS EN 645,
TS EN 647

Plastic Based Substances and Materials in Contact with Food

Determination of Formaldehyde
Spectrophotometric Method

EUR 24815, TS CEN/TS 13130 -23

Paper Based Packaging

Determination of Formaldehyde
Spectrophotometric Method

EN 645/ EN 647
TS EN 1541

Food

Enumeration of Coliform Bacteria, Fecal Coliform and Escherichia coli
MPN Technique - Colony Count Technique

FDA-BAM Chapter 4

Bread Yeast

Enumeration of Spores of B.subtilis

TS 3522

Food
Feed
Environmental Samples from Food Premises

Detection of Salmonella spp.

TS EN ISO 6579-1

Milk and Milk Products,
Powdered Baby Food

Detection of Cronobacter spp.

ISO 22964

Food

Enumeration of Sulfite-Reducing Anaerobic Bacteria
Colony Count Technique

ISO 15213

Food

Detection of Campylobacter spp.

ISO 10272-1

Food Feed

Enumeration of Escherichia coli
Colony Count Technique

ISO 16649-2

Food
Feed
Environmental Samples from Food Premises

Detection of Listeria monocytogenes

ISO 11290-1

Food
Feed
Environmental Samples from Food Premises

Aerobic Plate Count
Pour Plate Technique

ISO 4833-1

Food

Enumeration of Bacillus cereus
Colony Count Technique

ISO 7932

Food

Enumeration of Clostridium perfringens
Colony Count Technique

ISO 7937

Food

Detection of Escherichia coli O157

ISO 16654

Food
Feed
Environmental Samples from Food Premises

Enumeration of Enterobactericea
Colony Count Technique

ISO 21528-2

Food
Feed
Environmental Samples from Food Premises

Enumeration of Coliform Bacteria
Colony Count Technique

ISO 4832

Food
Feed
Environmental Samples from Food Premises

Enumeration of Yeast and Mold
Colony Count Technique

ISO 21527-1
ISO 21527-2

Sea Food

Detection of Vibrio cholerae and Vibrio parahaemolyticus


ISO 21872-1

Food
Feed
Environmental Samples from Food Premises

Enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus and Other Species)
Colony Count Technique

ISO 6888-1

Food
Feed
Environmental Samples from Food Premises

Enumeration of Escherichia coli
MPN Technique

ISO 16649-3

Food
Feed
Environmental Samples from Food Premises

Detection of Salmonella spp.

AFNOR BRD 07/11-12/05 RAPID’
Salmonella Agar-V10

Food
Feed
Environmental Samples from Food Premises

Detection of Listeria monocytogenes

AFNOR BRD 07/04-09/98 RAPID’
L.mono-V11

Milk and Milk Products,
Powdered Baby Food

Detection of Cronobacter spp.

AFNOR BRD 07/22-05/12 RAPID’
Sakazakii Agar-V3

Bread and Bread Types

Enumeration of Spores of B.subtilis

TS 5000

Food
Feed
Environmental Samples from Food Premises

Enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus and Other Species)
MPN Technique

ISO 6888-3

Food
Feed
Environmental Samples from Food Premises

Enumeration of Yeast and Mold
(aw ≤0,95 and aw>0,95)                                                                 Colony Count Technique

RAPID Yeast and Mold Count Plate – 3M                                                            AFNOR Certifica No: 01/13 – 07/14)

Food
Feed
Environmental Samples from Food Premises

Enumeration of Enterobacteriaceae
MPN Technique

ISO   21528-1

Sugar and Sugary Products

Enumeration of Osmophilic Yeast
Colony Counting Technique

ISO 21527-2

Food

Detection of Staphylococcal Enterotoxin
ELISA Method

EU-RL European Screening Method
Ver.5 (RIDASCREEN Set Total Kit R
4105)

Yoghurt

Enumeration of Characteristic Microorganism in Yogurt
Colony Counting Technique

ISO 7889

Food

Enumeration of Lactic Acid Bacteria
Colony Counting Technique

ISO 15214

Food Feed

GMO Screening Analysis
(p35S /tNOS /pFMV)
Real Time PCR Method
Pretreatment: DNA Extraction

EURL Method (QL-ELE-00-012),EURL Method (QL-ELE-00-015), ISO21569, ISO 24276, Kit Method(Magnesia 16 Nucleic Acid Isolation KitManual), ISO 21571

Food

Determination of Meat Species (" Horse, Donkey" Meat Search) "Conventional PCR" Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.006 / 03"
(Modified from Kesmen, Z., Sahin, F., and Yetim, H., PCR assay for the identification of animal species in cooked sausages, Meat Sci, 77 (4), 649-653.)
Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Determination of Meat Type ("Chicken, Turkey" Meat Search) "Conventional PCR" Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.008 / 03" (I.
Martin, T. Garcia, V.Fajardo, I. Lopez-Calleja, M. Rojas, M.A. Pavon et al. Technical Note: Detection of Chicken, Turkey, Duck and Goose Tissues in Feedstuffs using species-specific polymerase chain reaction, Journal of Animal Science, 85 (2007). pp. Modified from 452-458) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Species Identification (Beef DNA Search)
Real Time PCR Method
Pre-Process:DNA Extraction

Kit Method (Exon Screen Beef Assay) ,
ISO 21569, ISO 24276 Kit Method (Exon
Screen Pork Assay), ISO 21569, ISO
24276

Food
Feed

Species Identification (Pork DNA Search)
Real Time PCR Method
Pre-Process:DNA Extraction

Kit Method (Exon Screen Pork Assay),
ISO 21569, ISO 24276 Kit Method
(Magnesia 16 Nucleic Acid Isolation Kit Manual)

Gelatin and Gelatin
Containing Foods

Determination of Meat Species ("Pork, Beef," Fish Meat Search) "Conventional PCR" Method
Pretreatment: DNA Extraction

İşletme İçi Metot-
“MİKROKİM.SOP.04.021 Rev03” ( H. Cai., , X. Gu., M. S. Scanlan., D. H.
Ramatlapeng., C. R. Lively. Real-time
PCR Assays for Detection and
Quantitation of Porcine and Bovine DNA
in Gelatin Mixtures and Gelatin
Capsules- Sun, M., Liang, C., Gao, H.,
Lin, C., & Deng, M. (2009). Detection of
Parvalbumin, a Common Fish Allergen
Gene in Food, by Real-Time Polymerase Chain Reaction’den modifiye edilmiştir)
Kit Metodu (Magnesia 16 Nükleik Asit
İzolasyon Kit Manuel)

Meats and Foods Containing All Meat Products

Determination of Meat Species ("Duck" Meat Search) "Real Time PCR" Method

Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.019 / 02" (N.
Pegels., I. González., I.
López-Calleja., S. Fernández., T. García. R. Martín. Evaluation of a TaqMan Real-Time PCR Assay for Detection of Chicken,
Turkey, Duck, and Goose Material in Highly Processed Industrial Feed Samples, Departamento de Nutrición, Bromatología y Tecnología de los Alimentos Facultad de Veterinaria.)
Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Determination of Milk Type ("Sheep, Goat and Cow" Milk Search) Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.017 / 02"
(Restsch.J. et al. Modified from Eur Food Res Technol (2013) 236: 217-227) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Searching for Animal DNA (16S rRNA Gene) Conventional PCR Method Pretreatment: DNA Extraction

In-House Method -MİKROKİM.SOP.04.068 / 00 "
(Food Control 43 (2014)
35-41.), ISO 21569
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Searching Plant Specific "Soybean" Gene Real Time PCR Method
Pretreatment: DNA Extraction

EURL Method (QT-TAX-GM-009), ISO 21569,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Plant Specific Corn Gene Search Real Time PCR Method
Pre-treatment: DNA Extraction

EURL Method (QT-TAX-ZM-011), ISO 21569, ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual, ISO 21571)

Food
Feed

Plant Specific Canola Gene Search Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-TAX-BN-012), ISO 21569, ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Plant Specific Cotton Gene Search
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD (QT-TAX-GH-015), ISO 21569,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Plant Specific Rice Gene Search
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-TAX-OS-002), ISO 21569,
ISO 24276, Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Plant Specific Potato Gene Search Real Time PCR Method Pre-treatment: DNA Extraction

EURL Method (QT-TAX-ST-010), ISO 21569, ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Plant Specific Actin Gene Search Real Time PCR Method
Pre-treatment: DNA Extraction

In-House Method (MİKROKİM.SOP.04.014 / 02) '' (I.
Laube., H. Hird., P. Brodmann.,
S. Ullmann., M.
Schöne-Michling. J. Chisholm.
H. Broll.
Development of Primer and Probe Sets for the Detection of Plant Species in Honey, Food Chemistry
From 118 (2010) 979-986.
Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON87701 Soy Type Determination and Quantity Analysis Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GM-010), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON87708 Soy Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD (QT-EVE-GM-012), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 87769 Soy Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD (QT-EVE-GM-002), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

CV127 (BPS-CV127-9) Soy Type Determination and Quantity Analysis Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD,
(QT-EVE-GM-011), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

DP 305423-1 Soy Type Identification and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD (QT-EVE-GM-008), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

DAS 44406-6 Soy Type Identification Analysis Real Time PCR Pretreatment: DNA Extraction

EURL METHOD,
QT-EVE-GM-015, ISO 21570, ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

DAS 68416-4 Soy Type Identification Analysis Real Time PCR Pretreatment: DNA Extraction

EURL METHOD
QT-EVE-GM-013, ISO 21570, ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

DAS 81419-2 Soy Type Identification Analysis Real Time PCR Pretreatment: DNA Extraction

EURL METHOD,
QT-EVE-GM-014, ISO 21570, ISO 24276, Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

MON 87751-7 Soy Type Identification Analysis Real Time PCR Pretreatment: DNA Extraction

EURL METHOD QT-EVE-GM-016, ISO 21570, ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

DAS 40278-9 Corn Type Identification and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-004), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

73496 Canola Type Identification Analysis
Real Time PCR Method
Pretreatment: DNA Extraction

EURL METHOD (QT-EVE-BN-009), ISO
21569, ISO 24276 Kit Method
(Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

281-24-236 Cotton Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-001a), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

3006-210-23 Cotton Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-001b), ISO 21570
ISO 24276

Food
Feed

GHB614 Cotton Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-006), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

GHB 811 Cotton Type Determination Analysis
Real Time PCR Method
Pretreatment: DNA Extraction

EURL METHOD (QT-EVE-GH-013), ISO
21569, ISO 24276 Kit Method
(Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

H7-1 Beet Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD (QT-EVE-BV-001), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Bt11 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pretreatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-015), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 810 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-020), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 89034 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-018), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

MON88017 Maize Type Determination and Quantity Analysis Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-016), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MIR604 Maize Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-013), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual),
ISO 21571

Food
Feed

NK603 Corn Type Determination and Quantity Analysis Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-008), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MIR 162 Corn Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-022), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit
Manual), ISO 21571

Food
Feed

DAS 1507 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-010), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

3272 Corn Type Identification and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-019), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 863 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD,
(QT-EVE-ZM-009), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

DAS 59122 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-012), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

T25 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-011), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

GA21 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METOT (QT-EVE-ZM-007), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

Bt 176 Corn Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METOT (QT-EVE-ZM-023), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 87460 Corn Type Determination and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-ZM-005), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

40-3-2 Soy Type Identification and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GM-005), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

A2704-12 Pedigree Determination and Quantity Analysis Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METOT (QT-EVE-GM-004), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

A5547-127 Soy Type Identification and Quantity Analysis
Real Time PCR Method
Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GM-007), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON89788 Soy Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GM-006), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

DP 356043-5 Soy Type Identification and Quantity Analysis Real Time PCR Method Pretreatment: DNA Extraction

EURL METHOD
(QT-EVE-GM-009), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 87705 Soy Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GM-003), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

EH92-527-1 Potato Type Identification and Quantity Analysis Real Time PCR Method Pretreatment: DNA Extraction

EURL METHOD
(QT-EVE-ST-001), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

LLRice 62 Rice Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-OS-002), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 15985 Cotton Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-005), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 1445 Cotton Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-003), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 531 Cotton Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-004), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

LL 25 Cotton Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-002), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

T304-40 Cotton Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-009), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 88913 Cotton Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-007), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual), ISO 21571

Food
Feed

GT73 Canola Type Identification and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-BN-004), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

T45 Canola Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD (QT-EVE-BN-001), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit
Manual), ISO 21571

Food
Feed

MS8 Canola Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-BN-002), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

RF3 Canola Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-BN-003), ISO 21570,
ISO 24276

Food
Feed

MS1 Canola Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-BN-005), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

TOPAS 19/2 Canola Type Identification and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-BN-008), ISO 21570,
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food
Feed

MON 88302 Canola Type Determination and Quantity Analysis Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD (QT-EVE-BN-010), ISO 21570,
ISO 24276

Food
Feed

GHB119 Cotton Type Determination and Quantity Analysis
Real Time PCR Method Pre-treatment: DNA Extraction

EURL METHOD
(QT-EVE-GH-008), ISO 21570
ISO 24276 Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Gluten Determination
Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM SOP.04.018 / 02" (D. Zeltner,
M. Glomb., D. Maede (2009). Real-Time PCR Systems for the Detection of the Gluten-Cointaining Cereals Wheat, Spelt, Kamut, Rye, Barley and Oat. Eur Food Res Technol Eur Food Res Technol
(2009) 228: Modified from 321-330) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Gluten-Free and Gluten-Reduced Whole Foods

Gluten Determination
ELISA Method

R-Bipharm Ridascreen Gliadin / Art No: R7001

Food

Plant Specific Chickpea Gene Screening Conventional PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.020 / 02" (R.
Alary C. Buissonade., P. Joudrier., M., Gautier. Detection and Discrimination of Cereal and Leguminous Species in Chestnut Flour by Duplex PCR. Eur Food Res Technol (2007) 225: 427-434) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Pistachio Allergen Gene Search Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.016 / 02" (B.
Brezna et al. Modified from Eur Food Res Technol (2008) 228: 197-203.) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Searching for Almond Allergen Gene Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.016 / 02" (L.
Piknova et al. Eur Food Res Technol (2008)
Modified from 226: 1155-1158) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Walnut Allergen Gene Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.016 / 02" (B.
Brezna et al. Modified from Eur Food Res Technol (2006) 223: 373-377.) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Hazelnut Allergen Gene Search Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.016 / 02" (L.
Piknova et al. Eur Food Res Technol (2008)
Modified from 226: 1155-1158) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual

Food

Peanut Allergen Gene Search Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.016 / 02" (R.
Köppel et al. Modified from Eur Food Res Technol (2010) 230: 367-374) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Cashew Allergen Gene Search Real Time PCR Method
Pretreatment: DNA Extraction

In-house Method-
"MIKROKİM.SOP.04.016 Rev02"
(A.Ehlert et al. Food Anal. Methods
Modified from (2008) 1:136-143
Kit Method (Magnesia 16
Nucleic Acid Isolation Kit Manual)

Food

Sesame Allergen Gene Search Real Time PCR Method
Pretreatment: DNA Extraction

In-House Method "MİKROKİM.SOP.04.016 / 02" (S.
Mustorp et al. Modified from Eur Food Res Technol (2008) 226: 771-778.) Kit Method (Magnesia 16 Nucleic Acid Isolation Kit Manual)

Food

Allergen Detection (Searching for Milk Protein)
ELISA Method
Pretreatment: Protein Extraction

Kit Method(R-Biopharm Ridascreen Fast Milk/ Art No: R4652)

Food

Allergen Detection (Egg Protein Detection)
ELISA Method
Pretreatment: Protein Extraction

Kit Method (R-Biopharm Ridascreen Fast Ei/Egg Protein/Art No:R6402)

Water

Color Determination
Spectrophotometric Method

SM 2120 C

Water

pH Determination
Electrometric Method

TS EN ISO 10523

Water

Determination of Conductivity
Electrometric Method

TS 9748 EN 27888

Water

Determination of Nitrite / Nitrite Nitrogen
Spectrophotometric Method

TS 7526 EN 26777

Water

Determination of Ammonium / Ammonium Nitrogen
Pretreatment: Distillation Method
Measurement: Titrimetric Method

SM 4500 – NH3 B, C

Water

Determination of Chloride
Titrimetric Method

TS 4164 ISO 9297

Water

Determination of Free Chlorine
Spectrophotometric Method

TS EN ISO  7393-2

Water

Escherichia coli
Count
Membrane Filtration Technique

ISO 9308-1

Water

Coliform Bacteria Count
Membrane Filtration Technique

ISO 9308-1

Water

Counting Fecal Enterococci
Membrane Filtration Technique

TS EN ISO 7899-2

Water

Pseudomonas aeruginosa count
Membrane Filtration Technique

TS EN ISO 16266

Water

Sulfite-Reducing Anaerobic Bacteria (Clostridia) Spores Search and Count
Membrane Filtration Technique

TS 8020 EN 24461-2

Water

Colony Count (22 ⁰C)

TS EN ISO 6222

Water

Colony Count (36 ⁰C)

TS EN ISO 6222

Water

Detection and Enumeration of Pathogen Staphylococci
Membrane Filtration Technique

NF T90-412

Water

Clostridium perfringens Count (Including Spores)
Membrane Filtration Technique

98/83/EC Direktive

Water

Aluminium (Al), Arsenic (As), Copper (Cu), Mercury (Hg), Iron (Fe), Cadmium(Cd), Crom (Cr), Lead (Pb), Manganese (Mn), Nickel (Ni), Selenium (Se),Antimony (Sb), Sodium (Na), Calcium (Ca), Barium (Ba), Magnesium (Mg),Potasium (K), Boron (B)
ICP MS Method

EPA 3015 A EPA 6020 B